It is a monocotyledonous triploid fruit that belongs to the family Bromeliaceae. Pineapple ( Ananas comosus L.) is the second most economically important fruit crop in the world after banana, and the improvement of its qualities are aimed at increasing yield, taste and resistance to environmental changes. Conclusionīased on our findings, the presently described method is an efficient and effective strategy for pineapple protoplast isolation and transformation it is convenient and time saving and provides a greater platform for transformation studies. Further findings showed that the system is also suitable for protein–protein interaction studies. Additionally, colocalization analysis of two pineapple Mg 2+ transporter genes in pineapple protoplasts was consistent with the results in a tobacco transient expression system, confirming that the protoplast isolation method can be used to study subcellular localization.
The quality of the isolated protoplasts was verified using organelle localization in protoplasts with different organelle markers. Compared to previously reported protocols, our protoplast isolation method is markedly faster (saving 4.5 h), requires only a small quantity of tissue sample (1 g of leaves) and has high yield (6.5 × 10 5). A cocktail of 1.5% (W/V) Cellulase R-10 and 0.5% (W/V) Macerozyme R-10 resulted in 51% viable protoplasts with 3 h digestion. To address the above limitations, we have developed an efficient system for protoplast isolation and subcellular localization of desired proteins using pineapple plants derived from tissue culture.
This has limited the full exploration of the genetic repository of pineapple as well as the study of its genes, protein localization and protein interactions. Presently, however, quick and effective transformation systems are still lacking for many plant species including pineapple. A quick transformation system is also crucial for the functional analysis of genes along with the study of proteins and their interactions in vivo.
Ready to take your reading offline? Click here to buy this book in print or download it as a free PDF, if available.An efficient transformation protocol is a primary requisite to study and utilize the genetic potential of any plant species. View our suggested citation for this chapter. Share a link to this book page on your preferred social network or via email. To search the entire text of this book, type in your search term here and press Enter. Switch between the Original Pages, where you can read the report as it appeared in print, and Text Pages for the web version, where you can highlight and search the text. Also, you can type in a page number and press Enter to go directly to that page in the book. Jump up to the previous page or down to the next one. or use these buttons to go back to the previous chapter or skip to the next one. Show this book's table of contents, where you can jump to any chapter by name. Based on feedback from you, our users, we've made some improvements that make it easier than ever to read thousands of publications on our website.ĭo you want to take a quick tour of the OpenBook's features? No Thanks Take a Tour »
You're looking at OpenBook, NAP.edu's online reading room since 1999. Appendix F: Committee and Staff Biographical Information 249–254.
Appendix E: Scientific and Common Names 247–248.Appendix C: Committee on Genetically Modified Pest-Protected Plants Public Workshop 241–245.Appendix B: Example of Data Submitted to Federal Agencies 238–240.Appendix A: Costs of Regulating Transgenic Pest-Protected Plants 209–237.Strengths and Weaknesses of the Current Regulatory Framework 144–181 Crossroads of Science and Oversight 104–143 Potential Environmental and Human Health Implications of Pest-Protected Plants 40–103 Southern corn leaf blight and stalk rot fungus